ABSTRACT
SUMMARY: The geese's tongue filiform papillae are particularly long, and exhibit the same morphology of a tooth, evoking the lingual teeth of several fishes. In adult animals, they contain numerous mechanical Herbst's corpuscles but no taste buds. In the embryo, they appear since stage 38 and acquire their definitive shape between stages 38 and 42. They express several proteins associated with mammalian tooth development (BMP4, β-catenin, SHH, PITX2, PAX9), also known to be linked to parrot's pseudoteeth and goose's denticulations development. Neurofilaments are early present in the papillae primordia, and appear particularly numerous in adult papillae. Our results suggest that these papillae constitute a mechanical organ with a « tooth shape » derived from ancestral odontodes, whose development is controlled by numerous genes involved in classical odontogenesis.
Las papilas filiformes de la lengua de los gansos son particularmente largas y exhiben la morfología de un diente, evocando los dientes linguales presentes en varios peces. En los animales adultos, contienen numerosos corpúsculos de Herbst mecánicos, aunque una ausencia de papilas gustativas. En el embrión, aparecen a partir del estadio 38 y adquieren su forma definitiva entre los estadios 38 y 42. Expresan varias proteínas asociadas al desarrollo dentario de los mamíferos (BMP4, β-catenina, SHH, PITX2, PAX9), también conocidas por estar asociadas al desarrollo de pseudodientes en el loro y denticulaciones en el ganso. Los neurofilamentos están presentes tempranamente en los primordios de las papilas y aparecen particularmente numerosos en las papilas adultas. Nuestros resultados sugieren que estas papilas constituyen un órgano mecánico con «forma de diente» derivado de odontoides ancestrales, cuyo desarrollo está controlado por numerosos genes implicados en la odontogénesis clásica.
Subject(s)
Animals , Tongue/anatomy & histology , Tongue/metabolism , Geese/anatomy & histology , Tongue/embryology , Immunohistochemistry , Homeodomain Proteins , PAX9 Transcription Factor , Hedgehog Proteins , Bone Morphogenetic Protein 4ABSTRACT
OBJECTIVES@#Tongue squamous cell carcinoma (TSCC) is a common cancer in the oral and maxillofacial region, which seriously endangers people's life and health.Heterogeneous nuclear ribonucleoprotein A2/B1(hnRNP A2/B1) is an RNA-binding protein that regulates the expression of a variety of genes and participates in the occurrence and development of a variety of cancers. This study aims to investigate the role of hnRNP A2/B1 in TSCC progression.@*METHODS@#The differential expression of hnRNP A2/B1 in oral squamous cell carcinoma (OSCC) and normal oral mucosa cells and tissues was analyzed based on the gene expression profiles of GSE146483 and GSE85195 in the Gene Expression Omnibus (GEO) database. The correlation between hnRNP A2/B1 expression and disease-free survival of TSCC patients was analyzed based on TSCC related chip of GSE4676. TSCC cancer and paracancerous tissue samples of 30 patients were collected in Hunan Cancer Hospital from July to December 2021. Real-time RT-PCR and Western blotting were used to verify the mRNA and protein expression of hnRNP A2/B1 in TSCC patients'samples, respectively. Human TSCC Tca-8113 cells were transfected with hnRNP A2/B1 empty vector (a sh-NC group), knockdown plasmid (a sh-hnRNP A2/B1 group), empty vector overexpression plasmid (an OE-NC group) and overexpression plasmid (an OE-hnRNP A2/B1 group), respectively. The knockdown or overexpression efficiency of hnRNP A2/B1 was detected by Western blotting. The proliferation activity of Tca-8113 cells was detected by cell counting kit-8 (CCK-8), and the apoptosis rate of Tca-8113 cells was detected by flow cytometry.@*RESULTS@#Based on the analysis of OSCC-related chips of GSE146483 and GSE85195 in the GEO database, it was found that hnRNP A2/B1 was differentially expressed in the OSCC and normal oral mucosa cells and tissues (all P<0.01). Meanwhile, the analysis of TSCC related chip GSE4676 confirmed that the expression of hnRNP A2/B1 was negatively correlated with the disease-free survival of TSCC patients (P=0.006). The results of real-time RT-PCR and Western blotting showed that the relative expression levels of hnRNP A2/B1 mRNA and protein in TSCC tissues were significantly up-regulated compared with those in adjacent tissues (all P<0.01). The results of Western blotting showed that the expression level of hnRNP A2/B1 in Tca-8113 cells was significantly inhibited or promoted after knockdown or overexpression of hnRNP A2/B1 (all P<0.01). The results of CCK-8 and flow cytometry showed that inhibition of hnRNP A2/B1 expression in Tca-8113 cells reduced cell proliferation activity (P<0.05) and increased cell apoptic rate (P<0.01). Overexpression of hnRNP A2/B1 in Tca-8113 cells significantly increased cell proliferation (P<0.05) and decreased cell apoptosis (P<0.01).@*CONCLUSIONS@#HnRNP A2/B1 is a key factor regulating the proliferation and apoptosis of TSCC cells. Inhibition of hnRNP A2/B1 expression can reduce the proliferation activity of TSCC cells and promote the apoptosis of TSCC cells.
Subject(s)
Humans , Carcinoma, Squamous Cell/genetics , Sincalide/metabolism , Tongue Neoplasms/genetics , Mouth Neoplasms , Heterogeneous-Nuclear Ribonucleoprotein Group A-B/metabolism , RNA, Messenger , Tongue/metabolism , Cell Line, TumorABSTRACT
Abstract Acetaldehyde, associated with consumption of alcoholic beverages, is known to be a carcinogen and to be related to the tongue dorsum. Objective The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and bacterial characteristics on the tongue dorsum. Methodology Thirty-nine healthy volunteers participated in the study. Acetaldehyde concentrations in mouth air were evaluated by a high-sensitivity semiconductor gas sensor. A 16S rRNA gene sequencing technique was used to compare microbiomes between two groups, focusing on the six samples with the highest acetaldehyde concentrations (HG) and the six samples with lowest acetaldehyde concentrations (LG). Results Acetaldehyde concentration increased in correlation with the increase in bacterial count (p=0.048). The number of species observed in the oral microbiome of the HG was higher than that in the oral microbiome of the LG (p=0.011). The relative abundances of Gemella sanguinis, Veillonella parvula and Neisseria flavescens in the oral microbiome of the HG were higher than those in the oral microbiome of the LG (p<0.05). Conclusion Acetaldehyde concentration in mouth air was associated with bacterial count, diversity of microbiome, and relative abundance of G. sanguinis, V. parvula, and N. flavescens.
Subject(s)
Humans , Male , Female , Adult , Young Adult , Tongue/microbiology , Microbiota , Acetaldehyde/analysis , Mouth/surgery , Reference Values , Bacteria/isolation & purification , Bacteria/genetics , Tongue/metabolism , Candida/isolation & purification , Alcohol Drinking/metabolism , RNA, Ribosomal, 16S/analysis , RNA, Ribosomal, 16S/metabolism , Smoking/metabolism , Cross-Sectional Studies , Surveys and Questionnaires , Statistics, Nonparametric , Bacterial Load , Japan , Acetaldehyde/metabolism , Mouth/metabolismABSTRACT
Objective Acetaldehyde is the first metabolite of ethanol and is produced in the epithelium by mucosal ALDH, while higher levels are derived from microbial oxidation of ethanol by oral microflora such as Candida species. However, it is uncertain whether acetaldehyde concentration in human breath is related to oral condition or local production of acetaldehyde by oral microflora. The aim of this pilot study was to investigate the relationship between physiological acetaldehyde concentration and oral condition in healthy volunteers. Material and Methods Sixty-five volunteers (51 males and 14 females, aged from 20 to 87 years old) participated in the present study. Acetaldehyde concentration in mouth air was measured using a portable monitor. Oral examination, detection of oral Candida species and assessment of alcohol sensitivity were performed. Results Acetaldehyde concentration [median (25%, 75%)] in mouth air was 170.7 (73.5, 306.3) ppb. Acetaldehyde concentration in participants with a tongue coating status score of 3 was significantly higher than in those with a score of 1 (p<0.017). After removing tongue coating, acetaldehyde concentration decreased significantly (p<0.05). Acetaldehyde concentration was not correlated with other clinical parameters, presence of Candida species, smoking status or alcohol sensitivity. Conclusion Physiological acetaldehyde concentration in mouth air was associated with tongue coating volume. .
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Acetaldehyde/analysis , Mouth/chemistry , Tongue/chemistry , Acetaldehyde/metabolism , Candida albicans/isolation & purification , Cross-Sectional Studies , Ethanol/metabolism , Microbiota , Mouth Breathing/metabolism , Mouth Breathing/microbiology , Mouth/metabolism , Mouth/microbiology , Surveys and Questionnaires , Reference Values , Sex Factors , Statistics, Nonparametric , Time Factors , Tongue/metabolism , Tongue/microbiologyABSTRACT
BACKGROUND & OBJECTIVES: Malodour has been correlated with the concentration of volatile sulphur compounds produced in the oral cavity by metabolic activity of bacteria colonizing the periodontal sites and the dorsum of the tongue. The aim of this study was to detect malodour in mouth air organoleptically and using a portable sulphide monitor and to correlate it with the clinical parameters, halitosis linked toxins and BANA, using tongue and subgingival plaque samples. The halitosis grading is also correlated with the microbial colonies of the subgingival plaque sample. METHODS: 20 patients with chronic periodontitis with 5-7 mm pocket depth, radiographic evidence of bone loss and presence of oral malodour participated in this study. Assessment of mouth air was done organoleptically and by using a portable sulphide monitor. The clinical parameter, plaque index (PI), gingival index (GI), gingival bleeding index (BI), were obtained from all the areas. Samples for BANA and to detect halitosis linked toxins were taken from the dorsal surface of the tongue and periodontal pockets ranging 5-7 mm. Halitosis related microbial colonies were identified using anaerobic culturing from the subgingival plaque. RESULTS: The scores of PI, GI, BI and sample that tested positive for halitosis linked toxins and with the halitosis grading were not significant. The presence of tongue coating and the halitosis grading and toxin levels were significant. BANA has shown to be non contributory due to technical problems. Anaerobic culture has shown to identify Streptococcus, Bacteroides, Fusobacterium, Porphyromonas and Prevotella colonies. INTERPRETATION & CONCLUSION: The results confirmed that there was no correlation between the clinical parameters, halitosis linked toxins and halitosis grading. The microbial colonies have shown to correlate with the presence of oral malodour.
Subject(s)
Adult , Alveolar Bone Loss/metabolism , Bacterial Toxins/analysis , Bacteroides/isolation & purification , Benzoylarginine-2-Naphthylamide/diagnosis , Chronic Disease , Coloring Agents/diagnosis , Dental Plaque/chemistry , Dental Plaque Index , Female , Fusobacterium/isolation & purification , Gingival Hemorrhage/metabolism , Halitosis/diagnosis , Humans , Male , Middle Aged , Periodontal Index , Periodontal Pocket/metabolism , Periodontitis/complications , Porphyromonas/isolation & purification , Prevotella/isolation & purification , Streptococcus/isolation & purification , Sulfides/analysis , Tongue/metabolismABSTRACT
La patología de la lengua en el SIDA comprende un amplio espectro de lesiones tanto infecciosas como neoplasmas, que se presentan generalmente en estadios tempranos de la enfermedad y con mucha frecuencia en pacientes asintomáticas portadores de VIH, por lo cual se deduce la importancia de su reconocimiento precoz. Sin embargo, a menudo estas lesiones pasan desapercibidas o son confundidas con otras entidades, de modo que su incidencia real no se conoce. En el presente trabajo estudiamos las características macroscópicas, histoquímicas, inmunohistoquímicas y ultraestructurales de 30 lenguas de autopsias a pacientes con SIDA. Encontramos que el 100 de los especímenes presentó infecciones oportunistas, siendo la más frecuente la candidiasis, que se observó en 50 de los casos, seguidos por la leucoplasia vellosa (9 casos), infección por VPH (8 casos), Herpes simplex (4 casos), Histoplasma capsulatum (3 casos) y citomegalovirus (3 casos). En 6 especímenes encontramos características histopatológicas compatibles con enfermedad de Heck, condición que no había sido descrita anteriormente en pacientes con SIDA. En cuanto a las neoplasias, dos de los casos presentaron Sarcoma Kaposi y uno angiomatosis, todos ellos asociados a infección por CMV. Nuestros resultados inmunohistoquímicos con anti-LMP1 de EBV y el estudio ultraestructural
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Anatomy , Histocytochemistry , Immunohistochemistry , Pathology , Acquired Immunodeficiency Syndrome/pathology , Tongue/metabolism , Tongue/pathology , Tongue/ultrastructureABSTRACT
There is a strong correlation between gingival inflammation and clinical and sub-clinical ascorbic acid deficiency. This has created a demand for a handy diagnostic test to detect the ascorbic acid deficiency. The aim of this work was to find out the efficacy of one such test, i.e. the modified Lingual Ascorbic Acid Test (LAAT). To find out the significance of ths LAAT, it was compared with plasma ascorbic acid levels and then confirmed statistically. Our findings suggest that with this simple, reliable and inexpensive method, the dental practitioners can conveniently assess the ascorbic acid status of their patients.